Publications from Synbiosc - Lab for Synthetic Biology - Department of Engineering Science, University of Oxford
Publications
Peer-reviewed Articles
Paper Published (selected)
Synthetic Biology
ACS Synthetic Biology 2022 Mar 18;11(3):1349-1360. doi: 10.1021/acssynbio.1c00631.
Yu Y., Zhou J.X.Y., Li B., Ji M., Wang Y., Carnaby E., Andersson M.I., Huang W.E.*, Cui Z. (2022) A quantitative RT-qLAMP for the detection of SARS-CoV-2 and human gene in clinical application. Microbial Biotechnology 2022 Jul 13;. doi: 10.1111/1751-7915.14112.
Chen J.X., Lim, B., Steel, H., Song, Y., Ji, M., Huang, W.E.* (2021) Redesign of ultra-sensitive and robust gene circuit to sense DNA damage in probiotic E. coli Nissle 1917. Microbial Biotechnology Nov;14(6):2481-2496. doi: 10.1111/1751-7915.13767.
Chen X.J., Wang B., Thompson I.P., Huang W.E.* (2021) Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells. ACS Synthetic Biology 2021 Oct 15;10(10):2566-2578. doi: 10.1021/acssynbio.1c00223.
Zeng H., Rohani R., Huang W.E., Yang A. (2021) Understanding and mathematical modelling of cellular resource allocation in microorganisms: a comparative synthesis. BMC Bioinformatics 2021 Sep 28;22(1):467. doi: 10.1186/s12859-021-04382-3.
Martin L.K., Huang W.E., Thompson I.P. (2021) Bacterial wax synthesis. Biotechnology Advances 2021 Jan-Feb;46:107680. doi: 10.1016/j.biotechadv.2020.107680.
Soldan R., Sanguankiattichai N., Bach-Pages M., Bervoets I., Huang W.E., Preston G.M. (2020) From Macro to Micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization. Environmental Microbiology 2020 Oct 26;. doi: 10.1111/1462-2920.15296.
Huang W.E.*, Lim B., Hsu C.C., Xiong D., Wu W., Yu Y., Jia H., Wang Y., Zeng Y., Ji M., Chang H., Zhang X., Wang H., Cui Z. (2020) RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2. Microbial Biotechnology 13:950-961.
Cui Z., Chang H., Wang H., Lim B., Hsu C.C., Yu Y., Jia H., Wang Y., Zeng Y., Ji M., Liu W., Inverarity C., Huang W.E. (2020) Development of a rapid test kit for SARS-CoV-2: an example of product design. Bio-design and Manufacturing 11:83-86.
Catherine Fan, Paul A. Davison, Robert Habgood, Christoph M. Decker, Manuela Gesell Salazar, Khemmathin Lueangwattanapong, Helen E. Townley, Ian P. Thompson, Hua Ye, Zhanfeng Cui, Frank Schmidt, C. Neil Hunter and Wei E. Huang* (2020) Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology. Proceedings of the National Academy of Sciences of the United States of America 117:6752-6761.
Song Y., Cartron M.L., Jackson P.J., Davison P.A., Dickman M.J., Zhu D., Huang W.E.*, Hunter C.N. (2020) Overproduction of proteorhodopsin enhances long-term viability of Escherichia coli Applied & Environmental Microbiology 2020 86: e02087-19.
Fan C., Wu Y.H., Decker C., Rohani R., Gesell Salazar M., Ye H., Cui Z., Schmidt F., Huang W.E.*. (2019) Defensive Function of Transposable Elements in Bacteria. ACS Synthetic Biology 8:2141-2151.
Huang W.E. and Nikel P.I. (2019) The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back. Microbial Biotechnology 12:5-7.
Zhang Q., Song, Y., Amor, K., Huang, W.E., Porcelli, D., Thompson, I.P. (2019) Monitoring Cr toxicity and remediation processes-Combining a whole-cell bioreporter and Cr isotope techniques. Water Research 153:295-303.
Chen, J.X., Steel, H., Wu, Y-H, Wang, Y., Xu J., Rampley C.P.N., Thompson I.P., Papachristodoulou A, Huang W.E.*. (2019) Development of aspirin inducible biosensors in Escherichia coli and SimCells. Applied & Environmental Microbiology 85:e02959-18. Highlighted by AEM Spotlight.
Rampley, C.P.N., Davison, P.A., Qian, P., Preston, G.M., Hunter, C.N., Thompson, P.I., Wu, L.J., Huang, W.E.* (2017) Development of SimCells as a novel chassis for functional biosensors. Scientific Reports 7:7261.
Zhang D, Ding A, Cui S, Hu C, Thornton SF, Dou J, Sun Y, & Huang WE* (2013) Whole cell bioreporter application for rapid detection and evaluation of crude oil spill in seawater caused by Dalian oil tank explosion. Water Research 47(3):1191-1200.
Zhang D, Zhao Y, He Y, Wang Y, Zhao Y, Zheng Y, Wei X, Zhang L, Li Y, Jin T, Wu L, Wang H, Davison PA, Xu J & Huang WE* (2012) Characterization and Modeling of Transcriptional Cross-Regulation in Acinetobacter baylyi ADP1. ACS Synthetic Biology 1(7):274-283.
Zhang D, He Y, Wang Y, Wang H, Wu L, Aries E and Huang, WE.* (2012) Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microbial Biotechnology 5(1):87-97.
Wang Y, Chen Y, Zhou Q, Huang S, Ning K, Xu J, Kalin RM, Rolfe S & Huang WE* (2012) A Culture-Independent Approach to Unravel Uncultured Bacteria and Functional Genes in a Complex Microbial Community. PLoS One 7(10): (10): e47530.
Zhang D, Fakhrullin RF, Özmen M, Wang H, Wang J, Paunov VN, Li G & Huang WE* (2011) Functionalization of whole-cell bacterial reporters with magnetic nanoparticles. Microbial Biotechnology 4(1):89-97.
Rico A., Bennett M.H., Forcat S., Huang W.E. and Preston G.M. (2010) Agroinfiltration Reduces ABA Levels and Suppresses Pseudomonas syringae-Elicited Salicylic Acid Production in Nicotiana tabacum. PLOS One 5: e8977.
Song YZ, Li G, Thornton SF, Thompson IP, Banwart SA, Lerner DN and Huang WE* (2009) Optimization of Bacterial Whole Cell Bioreporters for Toxicity Assay of Environmental Samples. Environmental Science & Technology 43(20):7931-7938.
Huang WE*, Singer AC, Spiers AJ, Preston GM, & Whiteley AS (2008) Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environmental Microbiology 10(7):1668-1680.
Song YZ, Hahn T, Thompson IP, Mason TJ, Preston GM, Li GH, Paniwnyk L & Huang WE*. (2007) Ultrasound-mediated DNA transfer for bacteria. Nucleic Acids Research 35(e129).
Huang WE, Huang LF, Preston GM, Naylor M, Carr JP, Li YH. et al. (2006) Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp ADP1. Plant Journal 46(6):1073-1083.
Huang WE*, Wang H, Zheng HJ, Huang LF, Singer AC, Thompson IP & Whiteley AS (2005) Chromosomally located gene fusions constructed in Acinetobacter sp ADP1 for the detection of salicylate. Environmental Microbiology 7(9):1339-1348.
Single Cell Biology
Xu J., Yi X., Jin G., Peng D., Fan G., Xu X., Chen X., Yin H., Cooper J.M., Huang W.E.* (2022) High-Speed Diagnosis of Bacterial Pathogens at the Single Cell Level by Raman Microspectroscopy with Machine Learning Filters and Denoising Autoencoders. ACS Chemical Biology 2022 Feb 18;17(2):376-385. doi: 10.1021/acschembio.1c00834.
Liang P., Liu B., Wang Y., Liu K., Zhao Y., Huang W.E.*, Li B. (2022) Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology. Applied & Environmental Microbiology 2022 Feb 8;88(3):e0116521. doi: 10.1128/AEM.01165-21.
Zhang L., Li C., Peng D., Yi X., He S., Liu F., Zheng X., Huang W.E., Zhao L., Huang X. (2022) Raman spectroscopy and machine learning for the classification of breast cancers. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2022 Jan 5;264:120300. doi: 10.1016/j.saa.2021.120300.
Yi, X., Song, Y., Xu, X., Peng, D., Wang, J., Qie X., Lin K., Yu, M., Ge, M., Wang, Y., Zhang, D., Yang, Q., Wang, M., Huang W.E.* (2021) Development of Fast Raman Assisted Antimicrobial Susceptibility Test (FRAST) for antibiotic resistance analysis of clinical urine and blood samples. Analytical Chemistry 2021 Mar 30;93(12):5098-5106. doi: 10.1021/acs.analchem.0c04709.
Webb I.U.C., Xu J., Sánchez-Cañizares C., Karunakaran R., Ramachandran V.K., Rutten P.J., East A.K., Huang W.E., Watmough N.J., Poole P.S. (2021) Regulation and Characterization of Mutants of fixABCX in Rhizobium leguminosarum. Molecular Plant Microbe Interactions 2021 Oct;34(10):1167-1180. doi: 10.1094/MPMI-02-21-0037-R.
Xu J., Li X., Guo Z., Huang W.E., Cheng J.X. (2020) Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy. Analytical Chemistry 92:14459-14465.
Ng CK, Xu J., Cai Z, Yang L., Thompson IP, Huang W.E.*, Cao B. (2020) Elevated intracellular cyclic-di-GMP level in Shewanella oneidensis increases expression of c-type cytochromes. Microbial Biotechnology 13:1904-1916.
Hsu C.C., Xu J., Brinkhofa B., Wang H., Cui Z., Huang W.E.*, Ye H.* (2020) A Single-Cell Raman-based Platform to Identify Developmental Stages of Human Pluripotent Stem Cell-derived Neurons. Proceedings of the National Academy of Sciences of the United States of America 117: 18412-18423.
Wang Y., Xu J., Kong, K., Li B., Li H., Huang W.E.,* and Zheng C. Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota (2020) Environmental Microbiology 22:2613-2624.
Wang Y., Xu J., Kong K., Liu T., Yi L., Wang H., Huang W.E.* and Zheng C. Raman–DIP to study metabolic activities of single bacterial cells in human intestinal microbiota (2020) Microbial Biotechnology 13:572-583.
Xu J., Preciado-Llanes L., Aulicino A., Decker C.M., Depke M., Gesell Salazar M., Schmidt F., Simmons A., Huang W.E.*. (2019) Single-Cell and Time-Resolved Profiling of Intracellular Salmonella Metabolism in Primary Human Cells. Analytical Chemistry 91:7729-7737.
Liang, B., Gao, Y., Xu, J., Song, Y., Xuan, L., Shi, T., Wang, N., Hou, Z., Zhao, Y., Huang, W.E. and Chen, Z. (2019) Raman Profiling of Embryo Culture Medium to Identify Aneuploid and Euploid Embryos. Fertility and Sterility 111(4):753-762.
Zhang, S., Guo, L., Yang, K., Zhang, Y., Ye, C., Chen, S., Yu, X., Huang, W.E. and Cui L. (2018) Induction of Escherichia coli Into a VBNC State by Continuous-Flow UVC and Subsequent Changes in Metabolic Activity at the Single-Cell Level. Frontiers in Microbiology 9:2243.
Xu, J., Potter, M., Tomas, C., Elson, J., Morten, K.J., Poulton, J., Wang, N., Jin, H., Hou, Z., and Huang, W.E.* (2018) A new approach to find biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) by single-cell Raman micro-spectroscopy. Analyst 144:913-920.
Jing, X., Gou, H., Gong, Y., Su, X., Xu, L. Ji, Y. Song, Y., Thompson, I.P., Xu, J. Huang, W.E.* (2018) Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean. Environmental Microbiology 20:2241-2255.
Yuan, X., Song, Y., Song, Y., Xu, J., Wu, Y., Glidle, A., Cusack, M., Ijaz, U.Z., Cooper, J.M., Huang, W.E. and Yin, H. (2018) Effect of laser irradiation on cell function and its implications in Raman spectroscopy. Applied & Environmental Microbiology 84(8). pii: e02508-17.
Xu, J., Zhu, D., Ibrahim, A.D., Allen, C.R., Gibson, C.M. Fowler, P.W., Song, Y. Huang, W.E.* (2017) Raman-DIP (Deuterium Isotope Probing) reveals microbial metabolism at the single-cell level. Analytical Chemistry 89:13305-13312.
Song, Y., Cui, L., Lópezc, J.A.S., Zhu, Y.G., Thompson, I.P.,Xu, J., Huang, W.E.* (2017) Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River. Scientific Reports 7:16648. (F1000 recommended)
Cui, L., Yang, K., Zhou, G., Huang, W.E.*, Zhu, Y. (2017) Surface-enhanced Raman spectroscopy combined with stable isotope probing to monitor nitrogen assimilation at both bulk and single-cell level. Analytical Chemistry. 89:5793-5800.
Xu, J., Webb, I., Poole, P., Huang, W.E.* (2017) Label-free discrimination of Rhizobial bacteroids and mutants by single-cell Raman microspectroscopy. Analytical Chemistry 89: 6336−6340.
Tao, Y., Wang, Y., Huang, S., Zhu, P., Huang, W.E., Ling, J.Q., Xu, J. (2017) Metabolic-activity based assessment of antimicrobial effects by D2O-labeled Single-Cell Raman Microspectroscopy. Analytical Chemistry 89:4108-4115.
Song, Y., Kaster, A., Vollmers, J., Song, Y., Davison, P.A., Frentrup, M., Preston, G.M., Thompson, I.P., Murrell, J.C., Yin, H., Hunter, C.N., Huang, W.E.* (2017) Single cell genomics based on Raman sorting reveals novel carotenoid-containing bacteria in the Red Sea. Microbial Biotechnology. 10:125-137.
Teng, L., Wang, X., Wang, X., Ren, L., Wang, T., Wang, Y., Ji, Y., Huang, W.E.*, Xu, J. (2016) Label-free, rapid and quantitative phenotyping of stress response in E. coli via Ramanome. Scientific Reports. 6:34359.
Wang, Y., Song, Y., Tao, Y., Muhamadali, H., Goodacre, R., Zhou, N.Y., Preston, G.M., Xu, J., Huang, W.E.* (2016) Reverse and multiple stable isotope probing to study bacterial metabolism and interactions at the single cell level. Analytical Chemistry. 88: 9443-9450
Wang, Y., Huang, W.E.*, Cui, L. and Wagner, M. (2016) Single Cell Stable Isotope Probing in Microbiology using Raman Microspectroscopy. Current Opinion in Biotechnology 41:34-42.
Song, Y., Yin, H. and Huang, W.E.* (2016) Raman activated cell sorting. Current Opinion in Chemical Biology 33: 1-8.
Cui, L., Zhang, Y.J., Huang, W.E., Zhang, B., Martin,F.L.,Li, J., Zhang, K.S. and Zhu, Y.G. (2016) Surface-Enhanced Raman Spectroscopy for Identification of Heavy Metal Arsenic(V)-Mediated Enhancing Effect on Antibiotic Resistance. Analytical Chemistry 88:3164-3170.
McIlvenna, D., Huang, W.E., Davison, P.A., Glidle, A., Cooper, J.M. and Yin, H. (2016) Continuous cell sorting in a flow based on single cell resonance Raman spectra. Lab on a Chip 16:1420-1429.
Zhang P, Ren L, Zhang X, Shan Y, Wang Y, Ji Y, Yin H, Huang WE, Xu J, Ma B. (2015) Raman Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release. Analytical Chemistry 87:2282-2289.
Zhang D, Berry JP, Zhu D, Wang Y, Chen Y, Jiang B, Huang S, Langford H, Li G, Xu J, Aries E & Huang WE* (2015) Isolation and physiological characterisation of an uncultured degrader in a complex microbial community. ISME Journal 9:603-614.
Berry D, Mader E, Woebken D, Wang Y, Zhu D, Lee TK, Palatinszky M, Schintlmeister A, Schmid M, Shterzer N, Mizrahi I, Rauch I, Decker T, Bocklitzg T, Popp J, Gibson CM, Fowler PW, Huang WE, Wagner M (2015) Heavy water (D2O) incorporation as a universally applicable activity marker for microbial communities with single cell resolution. Proceedings of the National Academy of Sciences of the United States of America 112: 112:E194-203.
Huang WE*, Song Y, Xu J (2015) Single cell biotechnology to shed a light on biological 'dark matter' in nature. Microbial Biotechnology 8: 15-16.
Wang T, Ji Y, Wang Y, Jia J, Li J, Huang S, Han D, Hu Q, Huang WE* and Xu, J. (2014) Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopy. Biotechnology for Biofuels 7:58.
Wang Y, Ji Y, Wharfe ES, Meadows RS, March P, Goodacre R, Xu J, Huang WE* (2013) Raman Activated Cell Ejection for Isolation of Single Cells. Analytical Chemistry 85(22):10697-10701.
Li M, Huang WE, Gibson CM, Fowler PW, & Jousset A (2013) Stable Isotope Probing and Raman Spectroscopy for Monitoring Carbon Flow in a Food Chain and Revealing Metabolic Pathway. Analytical Chemistry 85(3):1642-1649.
Li MQ, Xu J, Romero-Gonzalez M, Banwart SA, & Huang WE (2012) Single cell Raman spectroscopy for cell sorting and imaging. Current Opinion in Biotechnology 23(1):56-63.
Li M, Canniffe DP, Jackson PJ, Davison PA, FitzGerald S, Dickman MJ, Burgess JG, Hunter CN & Huang WE* (2012) Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities. ISME Journal 6(4):875-885.
Li MQ, Ashok PC, Dholakia K, & Huang WE (2012) Raman-Activated Cell Counting for Profiling Carbon Dioxide Fixing Microorganisms. Journal of Physical Chemistry A 116(25):6560-6563.
Huang WE & Zhou JZ (2012) When single cell technology meets omics, the new toolbox of analytical biotechnology is emerging. Current Opinion in Biotechnology 23(1):1-1.
Huang WE*, Li MQ, Jarvis RM, Goodacre R, & Banwart SA (2010) Shining Light on the Microbial World: The Application of Raman Microspectroscopy. Advances in Applied Microbiology 70: 153-186.
Andrews JS, Rolfe SA, Huang WE, Scholes JD, & Banwart SA (2010) Biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species. Environmental Microbiology 12(9):2496-2507.
Huang WE*, Ward AD, & Whiteley AS (2009) Raman tweezers sorting of single microbial cells. Environmental Microbiology Reports 1(1):44-49.
Huang WE*, Ferguson A, Singer AC, Lawson K, Thompson IP, Kalin RM et al. (2009) Resolving Genetic Functions within Microbial Populations: In Situ Analyses Using rRNA and mRNA Stable Isotope Probing Coupled with Single-Cell Raman-Fluorescence In Situ Hybridization. Applied and Environmental Microbiology 75(1):234-241.
Huang WE, Stoecker K, Griffiths R, Newbold L, Daims H, Whiteley AS & and Wagner M (2007) Raman-FISH: combining stable-isotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function. Environmental Microbiology 9(8):1878-1889.
Huang WE*, Griffiths RI, Thompson IP, Bailey MJ, & Whiteley AS (2004) Raman microscopic analysis of single microbial cells. Analytical Chemistry 76(15):4452-4458.
Others
Xu A., Wu Y., Chen Z., Wu G., Ling F., Huang W.E. Hu H.Y. (2020) Towards the new era of wastewater treatment of China: Development history, current status, and future directions. Water Cycle 1: 80-87.
Rampley C.P.N., Whitehead P.G., Softley L., Hossain M.A., Jin L., David J., Shawal S., Das P., Thompson I.P., Huang W.E., Peters R., Holdship P., Hope R., Alabaster G. (2020) River toxicity assessment using molecular biosensors: Heavy metal contamination in the Turag-Balu-Buriganga river systems, Dhaka, Bangladesh. Science of the Total Environment 703:134760.
Timmis K, Cavicchioli R, Garcia JL, Nogales B, Chavarría M, Stein L, McGenity TJ, Webster N, Singh BK, Handelsman J, de Lorenzo V, Pruzzo C, Timmis J, Martín JLR, Verstraete W, Jetten M, Danchin A, Huang W, Gilbert J, Lal R, Santos H, Lee SY, Sessitsch A, Bonfante P, Gram L, Lin RTP, Ron E, Karahan ZC, van der Meer JR, Artunkal S, Jahn D, Harper L. (2019) The urgent need for microbiology literacy in society. Environmental Microbiology 21:1513-1528.
Blaud, A., Zaan, B., Menon, M., Lair, G.J. Zhang, D., Huber, P., Schiefer, J., Blum, W.E.H., Kitzler, B., Huang, W.E., van Gaans, P., Banwart, S. (2018) The abundance of nitrogen cycle genes and potential greenhouse gas fluxes depends on land use type and little on soil aggregate size. Applied Soil Ecology 125:1-11.
Wu, Y., Shen, L., Hu, H., Hankins, N.P., Huang, W.E.* (2018) An efficient microalgal biomass harvesting method with a high concentration ratio using the polymer-surfactant aggregates process. Algal Research 30:86-93.
Chao, H.J., Chen, Y.F., Fang, T., Xu, Y., Huang, W.E., Zhou, N.Y. (2016) HipH Catalyzes the Hydroxylation of 4-Hydroxyisophthalate to Protocatechuate in 2,4-Xylenol Catabolism by Pseudomonas putida NCIMB 9866. Applied and Environmental Microbiology 82: 724-731.
Meckenstock RU, Elsner M, Griebler C, Lueders T, Stumpp C, Aamand J, Agathos SN, Albrechtsen HJ, Bastiaens L, Bjerg PL, Boon N, Dejonghe W, Huang WE, Schmidt SI, Smolders E, Sørensen SR, Springael D, van Breukelen BM. (2015) Environmental Science & Technology 49: 7073-7081.
Perron GG, Lee AEG, Wang Y, Huang WE, & Barraclough TG (2012) Bacterial recombination promotes the evolution of multi-drug-resistance in functionally diverse populations. Proceedings of the Royal Society B-Biological Sciences 279(1733):1477-1484.
Huang WE*, Oswald SE, Lerner DN, Smith CC, & Zheng CM (2003) Dissolved oxygen imaging in a porous medium to investigate biodegradation in a plume with limited electron acceptor supply. Environmental Science & Technology 37(9):1905-1911.
Huang WE*, Smith CC, Lerner DN, Thornton SF, & Oram A (2002) Physical modelling of solute transport in porous media: evaluation of an imaging technique using UV excited fluorescent dye. Water Research 36(7):1843-1853.
Li G, Huang W*, Lerner DN, & Zhang X (2000) Enrichment of degrading microbes and bioremediation of petrochemical contaminants in polluted soil. Water Research 34(15):3845-3853.
Book Chapters (selected)
Wang, Y., Zhang, D., Davison, P.A. and Huang, W.E.* (2014) Bacterial whole cell biosensors for the detection of contaminants in water and soils. Methods in Molecular Biology – Environmental Microbiology 155-168.
Li, M., Boardman, D.G., Ward, A. and Huang, W.E.* (2014) Single-cell Raman sorting. Methods in Molecular Biology – Environmental Microbiology 147-153.
Wang, Y., Song, Y., Zhu, D., Ji, Y., Wang, Y., McIlvenna, D., Yin, H., Xu, J. and Huang W.E.* (2013) Probing and sorting single cells -the application of a Raman-activated cell sorter. Spectroscopy Europe 25: 16-20.
Song, Y., Zhang, D., Polizzi, K., Zhang, G.E., Li G. and Huang, W.E.* (2012) Bacterial whole cell bioreporters in environmental health. In Biosensors and Environmental Health, chapter 8:127-148.
Read, D.S., Huang, W.E., Whiteley, A.S. (2010) Raman-FISH. In: Handbook of Hydrocarbon and Lipid Microbiology. 4:4027-4038, Timmis, KN. ed. Springer.
Patents (selected)
- Developer of novel biosensors and gene trapping using Acinetobacter sp. ADP1 as a host, Patent No. WO2008/056144.
- Nucleic Acid Transfer Techniques, Patent number: GB2452543A.
- Biosensor and used thereof, Patent number: (2011) PCT/GB2011/052228, WO2012/066321A1.
- Construction of Raman Flow Cytometry and application on cell/particles separation. (2010) WO2012/055304.
- Alkane production - Biodiesel Synthesis by Bacteria, WO2013/128184
- Cell sorting WO 2017/144886.
- Biofilm transformation filed by Oxford University on 10/09/2019.
- Modified cell GB1915526.6, filed by Oxford University on 25/10/2019.
- Primers for detecting novel coronavirus SARCoV-2 and test kits, methods and applications Thereof, Chinese Patent Application No. CN202010232072.4, filed on 27th March 2020 (Oxford University Innovation project 17730).
- Switch design for stabilisation of LAMP reaction, Patent Application No. GB No. 2012480.6 (filing date 11 Aug 2020).