Method of obtaining optical sectioning by using structured light in a conventional microscope

OPT LETT 22 (24): 1905-1907 DEC 15 1997 Neil MAA, Juskaitis R, Wilson T

We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projectinga single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid areprocessed in real time to produce optically sectioned images that are substantially similar to those obtainedwith confocal microscopes.

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