Dr Gianfelice Cinque, Diamond Light Source - Rutherford Appleton Laboratory

Multimode InfraRed Imaging and Microspectroscopy at the Diamond Synchrotron
When Jun 08, 2015
from 02:00 PM to 03:00 PM
Contact Name
Contact Phone 01865-273925
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Infrared (IR) MicroSpectroscopy is a quantitative analytical method extensively applied for studying soft condensed matter because of its high molecular sensitivity and specificity. Specifically, Fourier Transform IR (FTIR) technique effectively probes optically-active vibrational modes of molecules, or IR fingerprint of molecular groups, at the microscopic scale. mFTIR combination with Synchrotron Radiation (SR) broadband and brightness provides an unique diffraction limited IR microprobe. In fact, SRIR photon flux density is up to 103 times higher than conventional sources and extends simultaneously from the near-IR (l > 1 mm) up to the far-IR (l < 1 cm). At MIRIAM beamline of Diamond such advantages are fully exploited to allow both the highest spatial resolution optically attainable in IR microscopy (practically dx ~ l fwhm), and an excellent spectral quality (figure of merit signal/noise>5000 rms in 30 sec) across the whole IR range for absorption spectroscopy.
 
The initial Life Science driver for MIRIAM of biochemical analysis of fixed cell cultures and tissue sections relevant to cancer, stem cell research and pathology, is now routine in confocal IR microscopy [1]. The new research in the field is ex vivo and real time IR microanalysis of living single cell, i.e. the study of subcellular metabolism via full field IR imaging e.g. imaging isotopic gradient around/inside living fibroblasts [2]. A new class of experiments have been pioneered at MIRIAM in the last couple of years, namely the in situ microanalysis of gas-solid interaction controlled by temperature which are specifically important for the chemistry of catalysis at single crystal level or functionalized Metal-Organic-Frames [3]. The recent optimization of the Coherent Synchrotron Radiation has expanded the MIRIAM experimental capability for absorption spectroscopy specifically in the “THz gap” domain. This is particular relevant in the study of large molecule collective modes e.g. the physics of MOFs [4], as well as the study of the water interaction with protein in solution [5].
 
1 A.J. Deegan et al. Analytical and Bioanalytical Chemistry 407 (2015) 1097, Tracking calcification in tissue-engineered bone using synchrotron micro-FTIR and SEM
2 L. Quaroni et al, Biophysical Chemistry 189 (2014) 40, Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium
3 A. Greenaway et al. Angewandte Chemie 126 (2014) 13701, In situ Synchrotron IR Microspectroscopy of CO2 Adsorption on Single Crystals of the Functionalized MOF Sc2(BDC-NH2)3†
4 M. R. Ryder et al.  Phys. Rev. Lett. 113 (2014) 215502, Identifying the Role of Terahertz Vibrations in Metal-Organic Frameworks: From Gate-Opening Phenomenon to Shear-Driven Structural Destabilization
5 J.W. Bye et al. J. Phys. Chem. A 118 (2014) 83, Analysis of the Hydration Water around Bovine Serum Albumin Using Terahertz Coherent Synchrotron Radiation

Speaker’s website: www.diamond.ac.uk/Beamlines/Soft-Condensed-Matter/B22/Staff/Cinque.html